These are the settings we used to calibrate the Samsung 32" N5300 (UN32N5300) TV. For gaming, some minor adjustments need to be done, and they are listed below.
The first thing we did was to verify that all of the Eco Solution settings from the General tab in the settings menu were disabled. When disabled, the TV's luminance won't change automatically depending on your room brightness.
For movies and TV content, we selected the 'Movie' Picture Mode, since it is the picture mode that gave us the closest result to our calibration goal and it should bring the image quality closest to what the content creator intended it to be.
We set the Picture Size to '16:9 Standard' with the Fit to Screen option set to 'On'. The Fit to Screen option allows you to fit and match the input signal without having to manually adjust the display scaling from the source output setting. It can also be useful to fully fill the screen when watching some lower resolution content, like old movies.
In our totally dark room and in order to have a luminosity close to our calibration target of 100 cd/m², we set the 'Backlight' to '16'. Note here that you should change Backlight to match your room lighting and it won't affect the picture quality in any way. We left the Brightness to '0', its default value, and the Contrast was set to '45'. We left the Sharpness to '0' to avoid any oversharpening and we left the Tint (G/R) to its default value of '0'.
We left Digital Clean View to 'Off' but if you are watching some older low-resolution content, you may want to set it 'On' to make the content smoother by introducing flicker. Note that this might be bothersome to some people and will lower the screen's brightness. Contrast Enhancer was left turned 'Off' since we don't want to add any extra image processing so we could see movies or any other content as it was intended. Film Mode was grayed out. It is only available when the TV receives a 480i, 720i or 1080i signal and it is supposed to help remove 24p judder. However, during our test, we were not able to remove 24p judder by enabling Film mode in these resolutions.
We selected the 'Warm2' Color Tone since it was the closest to our calibration goal, but you can change it to a cooler Color Tone if you find the color too yellowish or red. and we left Gamma to '0' as we do not wish to add any extra image processing. Color Space Setting should be left to 'Auto', as it will adapt depending on the source.
Update 01/03/2019: We incorrectly stated that the Color Space Setting should be set to 'Custom' for calibration. Unlike other Samsung TVs, there is no 'Custom' setting on the N5300. White balance and color changes apply to both 'Auto', and 'Native' color spaces.
For playing games, via a video game console or PC, you would need to enable Game Mode from the External Device Manager to have the lowest possible input lag.
If you are using your N5300 as a PC monitor and you want to enable the chroma subsampling (4:4:4), then set the input icon to 'PC' from the input menu if you want the TV to support chroma subsampling.
The following are the results of the white balance and colorspace calibration on our unit. They are provided for reference, and should not be copied as the calibration values vary per individual unit even for the same model and same size as the TV we reviewed due to manufacturing tolerances. If you want to try them you will need to enter all values shown, as all of them are active at the same time. If you end up with worse picture quality, simply reset them to the default values.